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Sterilization (microbiology)

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Moist heat sterilization A widely used method for heat sterilization is the autoclave , sometimes called a converter or steam sterilizer. To achieve sterility, the article is placed in a chamber and heated by injected steam until the article reaches a temperature and time setpoint. Almost all the air is removed from the chamber, because air is undesired in the moist heat sterilization process this is one trait that differs from a typical pressure cooker used for food cooking. The article is held at the temperature setpoint for a period of time which varies depending on what bioburden is present on the article being sterilized and its resistance D-value to steam sterilization. This may be achieved by gradually depressurizing the sterilization chamber and allowing liquids to evaporate under a negative pressure, while cooling the contents.
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